Category: Biochemical Engineering

200 Best MCQs on sterilization- biochemical engineering

Top 200 MCQs on sterilization- Biochemical engineering

This section contains MCQs/ Objective Type Questions of Sterilization – biochemical engineering for various competetive Exams like HPPSC HPSSC HPSSSB and other exams

Q1). The sterilization method depends

a).nature of additive
b).volume and feed rate
c).both (a) and (b)
d).none of these

View Answer

Correct Answer:
both (a) and (b)
Explaination:

Q2). The specific death rate of an organism can be expressed as

a).ln 2/D
b).D/ln2
c).D.ln2
d).2.0.3/ln2

View Answer

Correct Answer:
ln 2/D
Explaination:

Q3). The del factor (Δ) increases as the final number of cells

a).decreases
b).increases
c).zero
d).constant

View Answer

Correct Answer:
decreases
Explaination:

Q4). The filter material used for air filtration system is/are

a).glass wool
b).glass fibre
c).norite
d).all of the above

View Answer

Correct Answer:
all of the above
Explaination:

Q5). The deviation from ideal plug flow due to axial mixing can be described by the

a).dispersion model
b).Langmuir model
c).Friedlander model
d).Pasceri model

View Answer

Correct Answer:
dispersion model
Explaination:

Q6). The collection efficiency by diffusion increases with

a).decreasing particle size
b).decreasing air velocity
c).both (a) and (b)
d).increasing particle size

View Answer

Correct Answer:
both (a) and (b)
Explaination:

Q7). Glass fibers are favorable as a filter medium because they give a

a).lower pressure drop and are less liable to wetting or combustion
b).more pressure drop and are less liable to wetting or combustion
c).lower pressure drop and are more liable to wetting or combustion
d).more pressure drop and are more liable to wetting or combustion

View Answer

Correct Answer:
lower pressure drop and are less liable to wetting or combustion
Explaination:

Q8). The collection efficiency by interception increases with the

a).increasing particle diameter
b).increasing air flow velocity
c).both (a) and (b)
d).increasing particle size

View Answer

Correct Answer:
increasing particle size
Explaination:

200 Best MCQs on Agitation and aeration- biochemical engineering

Top 200 MCQs on agitation and aeration

This section contains MCQs/ Objective Type Questions of agitation and aeration – biochemical engineering for various competetive Exams like HPPSC HPSSC HPSSSB and other exams

Q1). Swirling and vortex formation can be prevented by

a).using baffles
b).using diffusion ring with turbines
c).both (a) and (b)
d).none of these

View Answer

Correct Answer:
both (a) and (b)
Explaination:

Q2). The power consumption in a stirring vessel can be assessed by using a

a).dynamometer
b).strain gauge attached to agitator shaft
c).watt meter attached to agitator motor
d).all of these

View Answer

Correct Answer:
all of these
Explaination:

Q3). Surface renewal theory predicts that the mass transfer coefficient is

a).directly proportional to the square root of the molecular diffusivity
b).inversely proportional to the square root of the molecular diffusivity
c).directly proportional to the cube root of the molecular diffusivity
d).indirectly proportional to the cube root of the molecular diffusivity

View Answer

Correct Answer:
directly proportional to the square root of the molecular diffusivity
Explaination:

Q4). The oxygen transfer rate in a bioreactor will increase if

a).oil is added
b).antifoam is added
c).detergent like molecules are added
d).increase in the reactor temperature

View Answer

Correct Answer:
detergent like molecules are added
Explaination:

Q5). Which of the following impellers are used for wide range of viscosities?

a).paddles
b).turbines
c).propellers
d).none of these

View Answer

Correct Answer:
turbines
Explaination:

Q6). Which of the following type(s) of stirrer can be used for microbiological processes?

a).Mig
b).Intermig
c).Turbine
d).All of these

View Answer

Correct Answer:
All of these
Explaination:

Q7). The range of width for the baffle in the fermenter is __________ times of vessel diameter

a).1/10 to 1/12
b).1/8 to 1/10
c).1/5 to 1/8
d).none of these

View Answer

Correct Answer:
1/10 to 1/12
Explaination:

Q8). The average concentration of oxygen in the boundary layers surrounding the bubbles (Co*) in a reactor is normally determined by

a).ultra-small dissolved oxygen probes
b).laser based photographic systems
c).measuring the steady state concentration of oxygen in the bulk liquid prior to inoculation of the reactor
d).measuring the steady state concentration of oxygen in the bulk liquid after inoculation of the reactor

View Answer

Correct Answer:
measuring the steady state concentration of oxygen in the bulk liquid prior to inoculation of the reactor
Explaination:

200 Best MCQs on Fermentation Reactors- biochemical engineering

Top 200 MCQs on Fermentation reactors

This section contains MCQs/ Objective Type Questions of fermentation reactors for various competetive Exams like HPPSC HPSSC HPSSSB and other exams

Q1). Yield coefficient represents

a).total biomass or product produced
b).conversion efficiency of a substrate into product
c).conversion rate of a substrate into biomass or product
d).production time of biomass or product

View Answer

Correct Answer:
conversion efficiency of a substrate into product
Explaination:

Q2). The lowest biomass yield in a culture of Escherichia coli will be in

a).an aerated batch culture containing a initial high concentration of glucose
b).an aerated batch reactor containing an initial low concentration of glucose
c).an aerated fed-batch reactor having a low glucose concentration
d).an aerated continuous reactor having a low glucose concentration

View Answer

Correct Answer:
an aerated batch culture containing a initial high concentration of glucose
Explaination:

Q3). When two populations compete for a single growth limiting substrate in a continuous fermenter, which organism would not be washed out?

a).Organism maintaining the highest substrate concentration
b).Organism maintaining the lowest substrate concentration
c).Both (a) and (b)
d).Organism maintaining the moderate substrate concentration

View Answer

Correct Answer:
Organism maintaining the lowest substrate concentration
Explaination:

Q4). The continuous cultures are not widely used in industry because

a).they are not suited for the production of secondary metabolites
b).contamination or mutation can have a disastrous effect on the operation
c).the government will not approve the licensing of pharmaceuticals produced in continuous cultures
d).all of the above

View Answer

Correct Answer:
all of the above
Explaination:

Q5). The lowest yield of ATP /is in

a).fermentation
b).aerobic respiration
c).anaerobic respiration
d).same in (a), (b) and (c)

View Answer

Correct Answer:
fermentation
Explaination:

Q6). Mixing in an anaerobic sludge blanket reactor is due to

a).rapid change in water temperatures throughout the reactor
b).release of gases by the microbial populations
c).swimming of microbes
d).none of the above

View Answer

Correct Answer:
release of gases by the microbial populations
Explaination:

Q7). Formation of end product by Lactococcus lactis will become non-growth associated as lactic acid accumulates because

a).cells will redirect ATP to anabolism
b).cells will redirect NAD+ to anabolism
c).cells will redirect ATP to facilitate the diffusion of lactic acid and H+ out of the cells
d).cells will redirect ATP to the active transport of lactic acid and H+ out of the cells

View Answer

Correct Answer:
cells will redirect ATP to the active transport of lactic acid and H+ out of the cells
Explaination:

Q8). Which of the following would not be subjected to the “glucose effect”?

a).Aspergillus fumigatus
b).Saccharomyces cerevisiae
c).Escherichia coli
d).Aspergillus niger

View Answer

Correct Answer:
Escherichia coli
Explaination:

200 Best MCQs on Water Treatment- biochemical engineering

Top 200 MCQs on Water Treatment

This section contains MCQs/ Objective Type Questions of Water Treatment for various competetive Exams like HPPSC HPSSC HPSSSB and other exams

Q1). The maximum desirable limit Bureau of Indian Standards (BIS) of lead in the drinking water is

a).0.05 mg/l
b).0.09 mg/l
c).0.1 mg/l
d).1.0 mg/l

View Answer

Correct Answer:
0.05 mg/l
Explaination:

Q2). Zeolite softening process removes

a).only temporary hardness of water
b).only permanent hardness of water
c).both temporary and permanent hardness of water
d).the dissolved gases in permanent hard water

View Answer

Correct Answer:
both temporary and permanent hardness of water
Explaination:

Q3). Conventional tertiary treatment is

a).chemical coagulation and flocculation
b).filtration
c).sedimentation
d).none of these

View Answer

Correct Answer:
chemical coagulation and flocculation
Explaination:

Q4). The maximum desirable limit (BIS) of total hardness (as CaCo3) in drinking water is

a).600 ppm
b).300 ppm
c).500 ppm
d).1000 ppm

View Answer

Correct Answer:
300 ppm
Explaination:

Q5). The chemical oxygen demand (COD)measures the

a).amount of oxygen required for growth of microorganisms in water
b).amount of oxygen that would be removed from the water in order to oxidize pollution
c).amount of oxygen required to oxidize the calcium present in waste water
d).none of the above

View Answer

Correct Answer:
amount of oxygen that would be removed from the water in order to oxidize pollution
Explaination:

Q6). Hardness of water does not

a).have any bad effect in boiler
b).make cooking of foods difficult
c).make it unfit for drinking
d).cause difficulty in the washing of clothes with soaps

View Answer

Correct Answer:
make it unfit for drinking
Explaination:

Q7). Permanent hard water may be softened by passing it through

a).sodium silicate
b).sodium bicarbonate
c).sodium hexametaphosphate
d).sodium phosphate

View Answer

Correct Answer:
sodium hexametaphosphate
Explaination:

Q8). Zeolite used in zeolite softening process for the treatment of hard water gets exhausted after certain time of usage but can be regenerated by flushing it with

a).10% calcium chloride solution
b).10% magnesium sulfate solution
c).10% magnesium chloride solution
d).10% sodium chloride solution

View Answer

Correct Answer:
10% sodium chloride solution
Explaination:

200 Best MCQs on Immobilized enzyme- biochemical engineering

Top 200 MCQs on Immbilized Enzyme

This section contains MCQs/ Objective Type Questions of Immobilized Enzyme for various competetive Exams like HPPSC HPSSC HPSSSB and other exams

Q1). Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have

a).low molecular weight
b).high molecular weight
c).high equivalent weight
d).low reactivity

View Answer

Correct Answer:
low molecular weight
Explaination:

Q2). Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are

a).free α-, β- or γ carb oxyl groups
b).α or β amino groups
c).phenyl, hydroxyl, sulfhydryl or imidazole groups
d).all of the above

View Answer

Correct Answer:
all of the above
Explaination:

Q3). Which medium is used for the production of Penicillin using immobilized cells

a).1% peptone medium
b).glucose medium
c).Yeast extract medium
d).LB broth

View Answer

Correct Answer:
glucose medium
Explaination:

Q4). Which of the following is taken as an assumption in the distributed model?

a).The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.
b).Mass transfer through the immobilized enzyme occurs via molecular diffusion
c).There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme
d).All of the above

View Answer

Correct Answer:
All of the above
Explaination:

Q5). The immobilized enzyme produced by micro encapsulation technique provides

a).an extremely large surface area
b).smaller surface area
c).high amount of solvent
d).relatively smaller surface area

View Answer

Correct Answer:
an extremely large surface area
Explaination:

Q6). Which of the following is considered as a disadvantage to the adsorption method of immobilization?

a).It is possible to separate and purify the enzymes while being immobilized
b).The enzymes are not usually deactivated by adsorption
c).The adsorption is a reversible process
d).State of immobilization is very sensitive to solution pH, ionic strength and temperature

View Answer

Correct Answer:
State of immobilization is very sensitive to solution pH, ionic strength and temperature
Explaination:

Q7). During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is

a).equal to that of substrate consumption
b).more than that of substrate consumption
c).lesser than that of substrate consumption
d).is nothing to do with the substrate consumption

View Answer

Correct Answer:
equal to that of substrate consumption
Explaination:

Q8). The immobilized technique involving chemical method is

a).covalent bond formation dependent
b).non-covalent bond formation dependent
c).both (a) and (b)
d).ionic bond formation dependent

View Answer

Correct Answer:
covalent bond formation dependent
Explaination:

200 Best MCQs on downstream processing- biochemical engineering

Top 200 MCQs on downstream processing

This section contains MCQs/ Objective Type Questions of downstream processing for various competetive Exams like HPPSC HPSSC HPSSSB and other exams

Q1). Concentration polarization can be reduced further by

a).pre filtering the solution
b).reducing the flow rate per unit membrane surface area
c).back washing periodically
d).all of the above

View Answer

Correct Answer:
all of the above
Explaination:

Q2). A system which require less solvent and produces a more concentrated extract phase, is desired with a

a).large distribution coefficients
b).small distribution coefficients
c).very small distribution coefficients
d).constant distribution coefficients

View Answer

Correct Answer:
large distribution coefficients
Explaination:

Q3). The efficiency of cell disruption in a bead mill depends on the

a).concentration of the cells
b).amount and size of beads
c).type and rotational speed of agitation
d).all of these

View Answer

Correct Answer:
all of these
Explaination:

Q4). A common filter medium is the cloth filter generally made of

a).canvas
b).synthetic fabrics
c).metal or glass fiber
d).all of these

View Answer

Correct Answer:
all of these
Explaination:

Q5). An ion exchange resin is composed of

a).polymeric network
b).ionic functional groups
c).counter ions
d).all of these

View Answer

Correct Answer:
all of these
Explaination:

Q6). Maintenance cost varies from

a).0-4% of the capital cost
b).12-16% of the capital cost
c).4-8% of the capital cost
d).8-12% of the capital cost

View Answer

Correct Answer:
4-8% of the capital cost
Explaination:

Q7). The optimum wet solid content for the cell suspension for a bead mill is typically somewhere between

a).5-15% by volume
b).15-30% by volume
c).30-60% by volume
d).60-90% by volume

View Answer

Correct Answer:
30-60% by volume
Explaination:

Q8). Filtration leaf test results indicate that the filtration rate of a protein product is 100 dry lbs/(ft^2 hr). What size production filter would be required to obtain 100 dry lbs of filter cake/hr?

a).1.54 ft^2
b).3.08 ft^2
c).65 ft^2
d).65 lbs/ ft^2.hr

View Answer

Correct Answer:
1.54 ft^2
Explaination:

200 Best MCQs on fluid flow- biochemical engineering

Top 200 MCQs on fluid flow

This section contains MCQs/ Objective Type Questions of fluid flow for various competetive Exams like HPPSC HPSSC HPSSSB and other exams

Q1). The viscosity of liquid was classified as both dilatant and rheopectic, which means that the viscosity

a).increased with stirring speed but decreased with mixing time
b).increased with stirring speed and increased with mixing time
c).decreased with stirring speed but increased with mixing time
d).decreased with stirring speed and decreased with mixing time

View Answer

Correct Answer:
increased with stirring speed and increased with mixing time
Explaination:

Q2). Which of the following describes the detrimental effect of bubbles on animal cells?

a).Cells trapped on the surface of bubbles can be damaged when the bubbles explode, releasing their pressure and surface energies
b).Cells trapped in the moving foam can be dragged apart by the bubbles
c).Both (a) and (b)
d).Bubbles can explode in the liquid medium causing the release of ultraviolet radiation

View Answer

Correct Answer:
Both (a) and (b)
Explaination:

Q3). A fluid in which the viscosity decreases with increasing stirrer speed and mixing time, can be represented as

a).newtonian fluid
b).pseudoplastic, thixotropic fluid
c).dilatant, rheopectic fluid
d).dilatant, pseudoplastic fluid

View Answer

Correct Answer:
pseudoplastic, thixotropic fluid
Explaination:

Q4). Filamentous fungal cells are more shear sensitive than bacterial cells because of

a).the presence of citin in cell walls of filamentous fungi
b).larger in size than bacterial cells and therefore are more susceptible to shear forces
c).fungal cells are much larger than bacterial cells
d).all of the above

View Answer

Correct Answer:
all of the above
Explaination:

Q5). The viscosity of water at STP is approximately

a).0.005 kg.m-1.s-1
b).0.001 kg.m-1.s-1
c).0.003 kg.m-2.s-1
d).0.1 g.cm-1.s-1

View Answer

Correct Answer:
0.001 kg.m-1.s-1
Explaination:

Q6). Bubble free aeration systems requires

a).silicone tubing or gas permeable membranes to facilitate gas transfer
b).carbon dioxide instead of air to supply oxygen
c).silicone tubing or gas permeable membranes to remove carbon dioxide by oxygen in the form of hydrogen peroxide
d).filled headspace of the reactor with carbon dioxide

View Answer

Correct Answer:
silicone tubing or gas permeable membranes to facilitate gas transfer
Explaination:

Q7). Non-linear relationship between shear stress and shear rate exists in

a).newtonian fluids
b).non-newtonian fluids
c).both (a) and (b)
d).none of these

View Answer

Correct Answer:
non-newtonian fluids
Explaination:

Q8). If the viscosity of a fluid decreases with increasing stirrer speed, then the nature of the fluid is

a).newtonian
b).pseudoplastic
c).dilatant
d).thixotropic

View Answer

Correct Answer:
pseudoplastic
Explaination:

200 Best MCQs on Fermentation kinetics- biochemical engineering

Top 200 MCQs on Fermentation Kinetics

This section contains MCQs/ Objective Type Questions of Fermentation Kinetics for various competetive Exams like HPPSC HPSSC HPSSSB and other exams

Q1). The function of the disengagement zone in an airlift fermenter is to

a).prevent CO2 rich bubbles from entering the downcomer
b).reduce the velocity of the bubbles
c).reduce liquid loss as aerosols
d).all of the above

View Answer

Correct Answer:
all of the above
Explaination:

Q2). Stationary phase is described as

a).no further increase in the cell population after a maximum value
b).deceleration of growth and division rate after the growth rate reaches a maximum
c).acceleration of growth and division rate after the growth rate reaches a maximum
d).deceleration of growth and division rate after the growth rate reaches a minimum

View Answer

Correct Answer:
no further increase in the cell population after a maximum value
Explaination:

Q3). In the accelerated phase, cell starts to

a).increase and the division rate increases to reach a maximum
b).decrease and the division rate increases to reach a maximum
c).increase and the division rate decreases to reach a maximum
d).increase and the division rate increases to reach a minimum

View Answer

Correct Answer:
increase and the division rate increases to reach a maximum
Explaination:

Q4). The monod model predicts that the specific growth rate

a).will increase with the concentration of the growth limiting substrate until it reaches a maximum value
b).will decrease with the concentration of the growth limiting substrate
c).will increase with the concentration of the growth limiting substrate
d).does not depend on growth limiting substrate

View Answer

Correct Answer:
will increase with the concentration of the growth limiting substrate until it reaches a maximum value
Explaination:

Q5). A mixed fermentation is one, which produces

a).both alcohol and carbon dioxide
b).both acid and carbon dioxide
c).both acid and alcohol
d).several different kinds of acid

View Answer

Correct Answer:
both acid and alcohol
Explaination:

Q6). The function of a mechanical seal is to

a).prevent contaminants entering the reactor
b).prevent cells from leaving the reactor
c).both (a) and (b)
d).prevent air to enter

View Answer

Correct Answer:
both (a) and (b)
Explaination:

Q7). The phenomenon in which substrates are used in a sequential manner is known as

a).trans-substrate genesis
b).dialism
c).diauxie
d).multiplicity

View Answer

Correct Answer:
diauxie
Explaination:

Q8). Diauxie is

a).growth factors
b).microbiological die off
c).the simultaneous uptake of nutrients
d).the stagewise uptake of nutrients

View Answer

Correct Answer:
the stagewise uptake of nutrients
Explaination:

200 Best MCQs on Enzymes and Applications- biochemical engineering

Top 200 MCQs on Enzymes and Applications

This section contains MCQs/ Objective Type Questions of Enzymes and Applications for various competetive Exams like HPPSC HPSSC HPSSSB and other exams

Q1). β-amylase is

a).endoenzyme
b).exoenzyme
c).saccharifying enzyme
d).both (b) and (c)

View Answer

Correct Answer:
both (b) and (c)
Explaination:

Q2). Juice clarification extraction is facilitated by using

a).cellulases
b).amylase
c).inulinase
d).lactase

View Answer

Correct Answer:
cellulases
Explaination:

Q3). Lysozyme is naturally present in

a).egg white
b).bacteria
c).tears & milk
d).all of these

View Answer

Correct Answer:
all of these
Explaination:

Q4). Enzymes act as antimicrobials

a).by depriving an organism of a necessary metabolite
b).by generating a substances toxic to the organism
c).by attracting a cell wall component
d).all of the above

View Answer

Correct Answer:
all of the above
Explaination:

Q5). Trichoderma β-glucanase is reported

a).to stabilize mashing
b).to convert taste fractions of dextrins to fermentable sugars in beer
c).to convert starch to dextrin
d).all of the above

View Answer

Correct Answer:
to stabilize mashing
Explaination:

Q6). The bitter taste of the high protein materials is reduced by using

a).invertase
b).dectinase
c).protease
d).none of these

View Answer

Correct Answer:
protease
Explaination:

Q7). Sulphydryl oxidase is used for

a).discoloration
b).clarification of images
c).UHT milk off flavour removal
d).all of these

View Answer

Correct Answer:
UHT milk off flavour removal
Explaination:

Q8). α-amylase is an endo enzyme which requires

a).Ca
b).Cu
c).Mn
d).None of these

View Answer

Correct Answer:
Ca
Explaination:

200 Best MCQs on Enzymes and kinetics- biochemical engineering

Top 200 MCQs on Enzymes and Kinetics

This section contains MCQs/ Objective Type Questions of Enzymes and Kinetics for various competetive Exams like HPPSC HPSSC HPSSSB and other exams

Q1). A competitive inhibitor of an enzyme is usually

a).a highly reactive compound
b).a metal ion such as Hg2+ or Pb2+
c).structurally similar to the substrate.
d).water insoluble

View Answer

Correct Answer:
structurally similar to the substrate.
Explaination:

Q2). Linear inhibition is sometimes called as

a).complete inhibition
b).incomplete inhibtion
c).partial inhibition
d).mixed inhibition

View Answer

Correct Answer:
complete inhibition
Explaination:

Q3). The types of inhibition pattern based on Michaelis Menten equation are

a).competitive
b).non-competitive
c).uncompetitive
d).all of the above

View Answer

Correct Answer:
all of the above
Explaination:

Q4). The effect of non-competitive inhibition on a Lineweaver-Burk Plot is that

a).it can move the entire curve to the right
b).it can change the y-intercept
c).it can change the x-intercept
d).all of these

View Answer

Correct Answer:
it can change the y-intercept
Explaination:

Q5). The rate-determining step of Michaelis Menten kinetics is

a).the complex formation step
b).the complex dissociation step to produce product
c).the product formation step
d).Both (a)and(c)

View Answer

Correct Answer:
the complex dissociation step to produce product
Explaination:

Q6). In competitive inhibition a factor is obtained from the measurement of

a).Vmax
b).Km
c).Y-intercept in Lineweaver-Burk Plot
d).None of these

View Answer

Correct Answer:
Km
Explaination:

Q7). Which of these proteases is not a cysteine active site protease?

a).Calpain
b).None of the above
c).Cathepsin D
d).Papain

View Answer

Correct Answer:
None of the above
Explaination:

Q8). The conformational change in an enzyme after the substrate is bound that allows the chemical reaction to proceed, can be explained by

a).induced fit
b).Pasteur
c).transition
d).fit and fine

View Answer

Correct Answer:
induced fit
Explaination: